Ecdysone receptor in the mud crab Scylla paramamosain: a possible role in promoting ovarian development

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Ecdysone receptor in the mud crab Scylla paramamosain: a possible role in promoting ovarian development.

In arthropods, it is known that ecdysteroids regulate molting, limb regeneration, and reproduction through activation of the ecdysone receptor (EcR). However, the ecdysteroid signaling pathway for promotion of ovarian development in crustaceans is still unclear. In this study, three cDNA isoforms of EcR were cloned from the mud crab Scylla paramamosain. qRT-PCR revealed that the SpEcR mRNA was ...

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Identification, Characterization, and Functional Analysis of Tube and Pelle Homologs in the Mud Crab Scylla paramamosain

Tube and Pelle are essential components in Drosophila Toll signaling pathway. In this study, we characterized a pair of crustacean homologs of Tube and Pelle in Scylla paramamosain, namely, SpTube and SpPelle, and analyzed their immune functions. The full-length cDNA of SpTube had 2052 bp with a 1578 bp open reading frame (ORF) encoding a protein with 525 aa. A death domain (DD) and a kinase do...

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Characterization and expression analysis of chymotrypsin after bacterial challenge in the mud crab, Scylla paramamosain

Chymotrypsin is one of the serine proteases families that have various biological functions. A chymotrypsin gene was isolated from hepatopancreas of the mud crab, Scylla paramamosain (designated SpCHY) in this study. The full-length cDNA of SpCHY contained 942 nucleotides with a polyadenylation sequence and encoded a peptide of 270 amino acids with a signal peptide of 17 amino acids. The SpCHY ...

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Rapid isolation and characterization of polymorphic microsatellite loci in the mud crab, Scylla paramamosain (Portunidae).

Scylla paramamosain is a widespread and commercially important species of coastal marine crab. We identified 13 polymorphic microsatellite loci from a genome library constructed with 5'-anchored PCR method. Thirty-two S. paramamosain from the East China Sea were used to analyze the characteristics of these loci. The number of alleles per locus ranged from 3 to 8, with a mean of 5.923. Observed ...

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The embryonic development of orange mud crab, Scylla olivacea (Herbst, 1796) held in the captivity

This study attempts to observe and record early embryonic developments of the orange mud crab, Scylla olivacea. The period taken by the eggs to hatch was 8 days and the colour of the eggs gradually changed from yellow to brown, gray and dark gray. During the embryonic development, the developing embryos reached the blastula stage within 24 hours with a mean egg diameter of 329.91 µm ± 6.62. The...

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ژورنال

عنوان ژورنال: Journal of Endocrinology

سال: 2015

ISSN: 0022-0795,1479-6805

DOI: 10.1530/joe-14-0526